PCR repeats 3 steps: denaturation 95°C strand separation → annealing ~55°C → extension 72°C (Taq). Each cycle the target copy number ×2 → after n cycles ≈ 2ⁿ copies. Only the stretch between the 2 primers is selectively amplified.
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Adjust ANNEALING TEMP & CYCLE COUNT on the right · pick a scenario (Run 2ⁿ · Sequencing · Gel · qPCR) · click “Cycle diagram” to see the 3 steps
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